Application

EIA and Western blots: 1:5,000-1:50,000.

Immunohistochemistry: 1:1,000-1:2,000.

Optimal working dilutions must be determined by end user.

Goat anti-Mouse IgG Antibody, Alkaline Phosphatase conjugate is an antibody against Mouse IgG for use in ELISA & WB.

Research Sub CategoryWhole Immunoglobulin Secondary Antibodies

Research CategorySecondary & Control Antibodies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Alkaline Phosphatase-conjugated Affinity Purified Goat anti-Mouse IgG (H+L)

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: AP126A

Physical form

Lyophilized. Buffer = 0.01 M Tirs HCl, 0.25 M NaCl, pH 8.0 with

15 mg/mL BSA and 0.05% sodium azide.

RECONSTITUTION:

Reconstitute with sterile distilled water to match the volume indicated on the vial label. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature.

ImmunoAffinity Purified

Specificity

Specific for mouse IgG, heavy and light chain.

Storage and Stability

this product is stable for several weeks at 2–8°C as an undiluted liquid. DO NOT FREEZE. For